Microarray和RNA-Seq数据的线性模型用户指南1 介绍2 先决条件limma软件包本身可以被引用为:(2015)limma为RNA测序和微阵列研究提供差异表达分析上面的文章回顾了limma....
2022-02-11 11:23:08 PUBLISHED
Chip-seq应用转录因子结合(TF-DNA binding)组蛋白修饰(Histone modification)数据分析流程原始数据QC、序列比对、过滤BAM、peak calling(MACS....
2022-01-29 13:22:59 PUBLISHED
cellranger输入文件:[Sample Name]_S1_L00[Lane Number]_[Read Type]_001.fastq.gzs20190405WTECP3_S1_L001_R1_....
2022-01-14 21:18:53 PUBLISHED
先决条件软件比对软件:STAR提取UMI和cell barcode:umi_tools质控fasta文件:cutadapt基因计数:featureCounts (安装subread)seuratCur....
2022-02-21 21:36:37 PUBLISHED
Single-Cell Analysis VS Bulk Analysis样本很少:早期受精卵每一个细胞都有不同的基因表达稀有细胞类型:干细胞很少,不能获得其特征异质性:肿瘤的亚型,发育中不同的状态单....
2022-01-16 18:20:08 PUBLISHED
网站功能免疫细胞浸润的程度与基因的表达是否相关the correlation of gene expression with immune cell infiltraion levelx轴:免疫细胞的....
2022-01-13 12:29:05 PUBLISHED
TopHat官网传送门1 双端测序reads的方向与转录本的方向问题传统的illumina测序,无法得知哪一个reads的方向与转录本方向一致,哪一个与转录本反向互补2 junction reads需....
2022-01-07 15:40:22 PUBLISHED
CXCR4miRNA(hsa-miR-655)https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0003677https://rnacentra....
2022-01-25 18:50:43 PUBLISHED
参考https://www.nature.com/articles/s41598-019-43935-8https://github.com/uc-bd2k/grein....
2021-08-20 10:14:49 PUBLISHED
基本概念GDSC: Genomics of Drug Sensitivity in Cancer,肿瘤细胞对药物的敏感性反应IC50(the half maximal inhibitory conce....
2021-08-18 17:30:25 PUBLISHED