扩增子16S下游分析流程
最后发布时间 : 2022-11-04 11:26:14
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经过之前的分析得到去除质体和非细菌的OTU表，并过滤特征表对应序列和特征表对应序列注释
results/otutab.txt
results/otus.fa
results/otus.sintax
otutab.txt
| #OTUID | KO1 | KO2 | KO3 | KO4 | KO5 | KO6 | OE1 | OE2 | OE3 | OE4 | OE5 | OE6 | WT1 | WT2 | WT3 | WT4 | WT5 | WT6 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ASV_1 | 382 | 671 | 282 | 438 | 381 | 390 | 476 | 590 | 500 | 360 | 490 | 357 | 799 | 830 | 567 | 664 | 508 | 519 |
| ASV_2 | 671 | 412 | 863 | 793 | 1032 | 631 | 245 | 169 | 414 | 440 | 348 | 239 | 440 | 536 | 267 | 386 | 497 | 424 |
| ASV_3 | 213 | 173 | 369 | 296 | 445 | 308 | 211 | 151 | 372 | 367 | 316 | 242 | 327 | 635 | 357 | 416 | 413 | 274 |
| ASV_4 | 506 | 141 | 181 | 257 | 480 | 178 | 203 | 196 | 134 | 210 | 214 | 129 | 362 | 473 | 196 | 192 | 394 | 290 |
| ASV_8 | 189 | 195 | 215 | 148 | 77 | 101 | 132 | 183 | 540 | 300 | 211 | 190 | 230 | 275 | 199 | 356 | 169 | 184 |
| ASV_6 | 302 | 232 | 307 | 309 | 490 | 365 | 89 | 95 | 114 | 143 | 134 | 137 | 178 | 191 | 154 | 184 | 158 | 158 |
otus.fa
>ASV_1
GTAGTCCACGCCGTAAACGGTGGGCGCTAGATGTGGGGACCTTCCACGGTTTCTGCGTCGCAGCTAACGCATTAAGCGCC
CCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTTGCTTA
ATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATCGCCGGAAAACTCGCAGAGATGCGGGGTCCTTTTGGGCCGG
TGACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCT
ATGTTGCCAGCACGCCCTTCGGGGTGGTGGGGACTCATAGGAGACTGCCGGGGTCAACTCGGA
>ASV_2
GTAGTCCACGCCCTAAACGATGTCAACTGGTTGTTGGGAGGGTTTCTTCTCAGTAACGTAGCTAACGCGTGAAGTTGACC
GCCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGTTTAAT
TCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGTCTGGAATCCTGAAGAGATTTGGGAGTGCTCGAAAGAGAGCC
AGAACACAGGTGCTGCATGGCCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGT
CATTAGTTGCTACGAAAGGGCACTCTAATGAGACTGCCGGTGACAAACCGGA
otus.sintax
| ASV_1 | d:Bacteria(1.00),p:Actinobacteria(1.00),c:Actinobacteria(1.00),o:Streptosporangiales(0.85),f:Thermomonosporaceae(0.80),g:Actinocorallia(0.48) | + | d:Bacteria,p:Actinobacteria,c:Actinobacteria,o:Streptosporangiales,f:Thermomonosporaceae,g:Actinocorallia |
|---|---|---|---|
| ASV_2 | d:Bacteria(1.00),p:Proteobacteria(1.00),c:Betaproteobacteria(1.00),o:Burkholderiales(1.00),f:Comamonadaceae(1.00),g:Pelomonas(1.00) | + | d:Bacteria,p:Proteobacteria,c:Betaproteobacteria,o:Burkholderiales,f:Comamonadaceae,g:Pelomonas |
| ASV_3 | d:Bacteria(1.00),p:Proteobacteria(1.00),c:Betaproteobacteria(1.00),o:Burkholderiales(1.00),f:Comamonadaceae(1.00),g:Rhizobacter(0.92) | + | d:Bacteria,p:Proteobacteria,c:Betaproteobacteria,o:Burkholderiales,f:Comamonadaceae,g:Rhizobacter |
| ASV_4 | d:Bacteria(1.00),p:Proteobacteria(1.00),c:Betaproteobacteria(1.00),o:Burkholderiales(1.00),f:Comamonadaceae(1.00),g:Rhizobacter(1.00) | + | d:Bacteria,p:Proteobacteria,c:Betaproteobacteria,o:Burkholderiales,f:Comamonadaceae,g:Rhizobacter |
| ASV_8 | d:Bacteria(1.00),p:Actinobacteria(1.00),c:Actinobacteria(1.00),o:Streptomycetales(1.00),f:Streptomycetaceae(1.00),g:Streptomyces(0.99) | + | d:Bacteria,p:Actinobacteria,c:Actinobacteria,o:Streptomycetales,f:Streptomycetaceae,g:Streptomyces |
| ASV_6 | d:Bacteria(1.00),p:Proteobacteria(1.00),c:Betaproteobacteria(1.00),o:Burkholderiales(1.00),f:Comamonadaceae(0.93),g:Piscinibacter(0.28) | + | d:Bacteria,p:Proteobacteria,c:Betaproteobacteria,o:Burkholderiales,f:Comamonadaceae,g:Piscinibacter |
| ASV_7 | d:Bacteria(1.00),p:Bacteroidetes(1.00),c:Flavobacteriia(1.00),o:Flavobacteriales(1.00),f:Flavobacteriaceae(1.00),g:Flavobacterium(1.00) | + | d:Bacteria,p:Bacteroidetes,c:Flavobacteriia,o:Flavobacteriales,f:Flavobacteriaceae,g:Flavobacterium |