扩增子16S上游分析流程
最后发布时间 : 2022-11-04 08:35:41
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测序数据
SampleID | Group | Date | Site | CRA | CRR | BarcodeSequence | LinkerPrimerSequence | ReversePrimer | Batch | Species | Sequencing | Platform | latitude_longitude | Description | fq1 | fq2 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KO1 | KO | 2017/6/30 | Chaoyang | CRA002352 | CRR117575 | ACGCTCGACA | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 39.59 N 116.22 E | Knock-out replicate 1 | KO1_1.fq.gz | KO1_2.fq.gz |
KO2 | KO | 2017/6/30 | Chaoyang | CRA002352 | CRR117576 | ATCAGACACG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 39.59 N 116.22 E | Knock-out replicate 2 | KO2_1.fq.gz | KO2_2.fq.gz |
KO3 | KO | 2017/7/2 | Changping | CRA002352 | CRR117577 | ATATCGCGAG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.42 E | Knock-out replicate 3 | KO3_1.fq.gz | KO3_2.fq.gz |
KO4 | KO | 2017/7/2 | Changping | CRA002352 | CRR117578 | CACGAGACAG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.42 E | Knock-out replicate 4 | KO4_1.fq.gz | KO4_2.fq.gz |
KO5 | KO | 2017/7/4 | Haidian | CRA002352 | CRR117579 | CTCGCGTGTC | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.21 E | Knock-out replicate 5 | KO5_1.fq.gz | KO5_2.fq.gz |
KO6 | KO | 2017/7/4 | Haidian | CRA002352 | CRR117580 | TAGTATCAGC | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.21 E | Knock-out replicate 6 | KO6_1.fq.gz | KO6_2.fq.gz |
OE1 | OE | 2017/6/30 | Chaoyang | CRA002352 | CRR117581 | TCTCTATGCG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 39.59 N 116.22 E | Over-expresion replicate 1 | OE1_1.fq.gz | OE1_2.fq.gz |
OE2 | OE | 2017/6/30 | Chaoyang | CRA002352 | CRR117582 | TACTGAGCTA | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 39.59 N 116.22 E | Over-expresion replicate 2 | OE2_1.fq.gz | OE2_2.fq.gz |
OE3 | OE | 2017/7/2 | Changping | CRA002352 | CRR117583 | CATAGTAGTG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.42 E | Over-expresion replicate 3 | OE3_1.fq.gz | OE3_2.fq.gz |
OE4 | OE | 2017/7/2 | Changping | CRA002352 | CRR117584 | CGAGAGATAC | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.42 E | Over-expresion replicate 4 | OE4_1.fq.gz | OE4_2.fq.gz |
OE5 | OE | 2017/7/4 | Haidian | CRA002352 | CRR117585 | ATACGACGTA | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.21 E | Over-expresion replicate 5 | OE5_1.fq.gz | OE5_2.fq.gz |
OE6 | OE | 2017/7/4 | Haidian | CRA002352 | CRR117586 | TCACGTACTA | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.21 E | Over-expresion replicate 6 | OE6_1.fq.gz | OE6_2.fq.gz |
WT1 | WT | 2017/6/30 | Chaoyang | CRA002352 | CRR117587 | CGTCTAGTAC | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 39.59 N 116.22 E | Wild-type replicate 1 | WT1_1.fq.gz | WT1_2.fq.gz |
WT2 | WT | 2017/6/30 | Chaoyang | CRA002352 | CRR117588 | TCTACGTAGC | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 39.59 N 116.22 E | Wild-type replicate 2 | WT2_1.fq.gz | WT2_2.fq.gz |
WT3 | WT | 2017/7/2 | Changping | CRA002352 | CRR117589 | CACGCGAGTC | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.42 E | Wild-type replicate 3 | WT3_1.fq.gz | WT3_2.fq.gz |
WT4 | WT | 2017/7/2 | Changping | CRA002352 | CRR117590 | ACGACTACAG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.42 E | Wild-type replicate 4 | WT4_1.fq.gz | WT4_2.fq.gz |
WT5 | WT | 2017/7/4 | Haidian | CRA002352 | CRR117591 | CGTAGACTAG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.21 E | Wild-type replicate 5 | WT5_1.fq.gz | WT5_2.fq.gz |
WT6 | WT | 2017/7/4 | Haidian | CRA002352 | CRR117592 | TACGAGTATG | AACMGGATTAGATACCCKG | ACGTCATCCCCACCTTCC | 1 | Arabidopsis thaliana | Novagene | HiSeq2500 | 40.10 N 116.21 E | Wild-type replicate 6 | WT6_1.fq.gz | WT6_2.fq.gz |
工具使用的数据库
- db: db