otus.fa
>ASV_1 GTAGTCCACGCCGTAAACGGTGGGCGCTAGATGTGGGGACCTTCCACGGTTTCTGCGTCGCAGCTAACGCATTAAGCGCC CCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTTGCTTA ATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATCGCCGGAAAACTCGCAGAGATGCGGGGTCCTTTTGGGCCGG TGACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCT ATGTTGCCAGCACGCCCTTCGGGGTGGTGGGGACTCATAGGAGACTGCCGGGGTCAACTCGGA >ASV_2 GTAGTCCACGCCCTAAACGATGTCAACTGGTTGTTGGGAGGGTTTCTTCTCAGTAACGTAGCTAACGCGTGAAGTTGACC GCCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGTTTAAT TCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGTCTGGAATCCTGAAGAGATTTGGGAGTGCTCGAAAGAGAGCC AGAACACAGGTGCTGCATGGCCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGT CATTAGTTGCTACGAAAGGGCACTCTAATGAGACTGCCGGTGACAAACCGGA
#结果有多个文件，需要目录 mkdir -p results/beta/ #基于OTU构建进化树 Make OTU tree, 4s usearch -cluster_agg results/otus.fa -treeout results/otus.tree #生成5种距离矩阵：bray_curtis, euclidean, jaccard, manhatten, unifrac usearch -beta_div results/otutab_rare.txt -tree results/otus.tree \ -filename_prefix results/beta/
results/beta/ ├── bray_curtis_binary.sorted.txt ├── bray_curtis_binary.tree ├── bray_curtis_binary.txt ├── bray_curtis.pcoa.label.pdf ├── bray_curtis.pcoa.pdf ├── bray_curtis.sorted.txt ├── bray_curtis.tree ├── bray_curtis.txt ├── euclidean.sorted.txt ├── euclidean.tree ├── euclidean.txt ├── jaccard_binary.sorted.txt ├── jaccard_binary.tree ├── jaccard_binary.txt ├── jaccard.sorted.txt ├── jaccard.tree ├── jaccard.txt ├── manhatten.sorted.txt ├── manhatten.tree ├── manhatten.txt ├── unifrac_binary.sorted.txt ├── unifrac_binary.tree ├── unifrac_binary.txt ├── unifrac.sorted.txt ├── unifrac.tree └── unifrac.txt