蛋白质一级结构是组成蛋白质多肽链的线性氨基酸序列。蛋白测序，蛋白序列分析，蛋白一级结构分析，蛋白氨基酸序列分析是确定蛋白质全部氨基酸序列的过程。通过蛋白质测序获得的信息，有非常多有价值的用途，包括：
- 用于蛋白质的鉴定；
- 设计分子克隆的探针；
- 合成可用作免疫原的肽段等。
蛋白测序服务常见有两种主要的方法：Edman降解（Harvey和Ferrier，2011； Bauer等，1997）和质谱分析（MS）（Sahukar等，2016）。
目前使用最广的蛋白质测序和鉴定方法是质谱法，而Edman降解则是表征蛋白质N端最重要的方法之一。
- PSMs:Displays the number of identified peptide spectrum matches identified from all included searches, including those redundantly identified.
蛋白表
| 主蛋白的识别号 | 基因名称 | 蛋白名称的详细描述 | 肽段覆盖率 | 鉴定到不同肽段的总数目 | 肽段匹配到二级谱图的数目 | unique肽段数目 | 归属的蛋白group数目 | 氨基酸残基数 | 蛋白的分子量 | 蛋白的等电点 | 定量值 | Sequest程序打分 |
|---|
| Accession | Gene Name | Description | Coverage | # Peptides | # PSMs | # Unique Peptides | # Protein Groups | # AAs | MW [kDa] | calc. pI | Area: F1: Sample | Score Sequest HT |
| Protein accession | Gene | Associated gene ID | Description | Coverage | # Proteins | Proteins in group | miR FASP_# Unique peptides | miR FASP_# Peptides | miR FASP_# PSMs | miR FASP_Protein error probability | miR FASP_q-value | miR FASP_PEP | miR FASP_MS1 precursor area | miR FASP_tmt10plex_126 | miR FASP_tmt10plex_126 - # quanted PSMs | miR FASP_tmt10plex_127N | miR FASP_tmt10plex_127N - # quanted PSMs | miR FASP_tmt10plex_127C | miR FASP_tmt10plex_127C - # quanted PSMs | miR FASP_tmt10plex_128N | miR FASP_tmt10plex_128N - # quanted PSMs | miR FASP_tmt10plex_128C | miR FASP_tmt10plex_128C - # quanted PSMs | miR FASP_tmt10plex_129N | miR FASP_tmt10plex_129N - # quanted PSMs | miR FASP_tmt10plex_129C | miR FASP_tmt10plex_129C - # quanted PSMs | miR FASP_tmt10plex_130N | miR FASP_tmt10plex_130N - # quanted PSMs | miR FASP_tmt10plex_130C | miR FASP_tmt10plex_130C - # quanted PSMs | miR FASP_tmt10plex_131 | miR FASP_tmt10plex_131 - # quanted PSMs |
|---|
| A2M | NA | ENSG00000175899 | alpha-2-macroglobulin [Source:HGNC Symbol;Acc:HGNC:7] | NA | na | NA | 8 | 9 | 12 | 2.874853755573355e-38 | 0.0 | 0.00162245 | 16352434.333333334 | 0.930830807074245 | 11 | 0.772021464969435 | 11 | 0.707903988854009 | 11 | 0.789387195411552 | 11 | 1.15137143542937 | 11 | 0.826996046675429 | 11 | 0.760178732847342 | 11 | 0.91701736567271 | 11 | 0.854543633421946 | 11 | 0.603476404140484 | 11 |
| A2ML1 | NA | ENSG00000166535 | alpha-2-macroglobulin-like 1 [Source:HGNC Symbol;Acc:HGNC:23336] | NA | na | NA | 1 | 1 | 1 | 0.0169213 | 0.0141899 | 0.7890064999999999 | 768887.0 | 0.887330152432326 | 1 | 1.09479780129344 | 1 | 1.0157434594774 | 1 | 1.29660702438037 | 1 | 0.973056657814277 | 1 | 0.997696101059528 | 1 | 1.26868409705732 | 1 | 1.13317687425116 | 1 | 1.33611556782666 | 1 | 1.12558143277205 | 1 |
| AAAS | NA | ENSG00000094914 | achalasia, adrenocortical insufficiency, alacrimia [Source:HGNC Symbol;Acc:HGNC:13666] | NA | na | NA | 6 | 6 | 11 | 8.178128730099644e-39 | 0.0 | 0.00162245 | 69955068.0 | 0.915436990896682 | 11 | 0.916836199551183 | 11 | 0.941698838939896 | 11 | 0.88544134353248 | 11 | 0.965729126460487 | 11 | 0.847425426889061 | 11 | 0.904473477822955 | 11 | 1.14627079478433 | 11 | 0.92015871221395 | 11 | 1.019089204302 | 11 |
| gene | miR.FASP_tmt10plex_126 | miR.FASP_tmt10plex_127N | miR.FASP_tmt10plex_127C | miR.FASP_tmt10plex_128N | miR.FASP_tmt10plex_128C | miR.FASP_tmt10plex_129N | miR.FASP_tmt10plex_129C | miR.FASP_tmt10plex_130N | miR.FASP_tmt10plex_130C | miR.FASP_tmt10plex_131 |
|---|
| A2M | 0.930830807074245 | 0.772021464969435 | 0.707903988854009 | 0.789387195411552 | 1.15137143542937 | 0.826996046675429 | 0.760178732847342 | 0.91701736567271 | 0.854543633421946 | 0.603476404140484 |
| AAAS | 0.915436990896682 | 0.916836199551183 | 0.941698838939896 | 0.88544134353248 | 0.965729126460487 | 0.847425426889061 | 0.904473477822955 | 1.14627079478433 | 0.92015871221395 | 1.019089204302 |
| AACS | 0.96245526191104 | 0.947590854699577 | 1.01977053925564 | 0.964200014652402 | 0.963690875567279 | 0.887846978098863 | 1.00208476389661 | 1.06062507803835 | 0.953661694404969 | 0.950616066145275 |
| AAED1 | 0.900508679946027 | 1.10751066220758 | 1.31041214292136 | 1.11527207267666 | 1.01450612064567 | 1.07518082408031 | 1.02966783708056 | 1.06466009392292 | 1.0366690039719 | 1.24507134739029 |
| AAGAB | 1.07230101858693 | 1.2126029407603 | 0.993777999029608 | 1.05762971893229 | 1.01511504613838 | 1.0637392532722 | 0.912021160518172 | 0.912052854266589 | 1.0656559070064 | 1.08498261123753 |
| AAK1 | 1.01350831210127 | 0.98433193366552 | 1.02797359945557 | 1.14908644786092 | 0.986735632115981 | 1.03463177873044 | 1.1678986196205 | 1.02933866306218 | 0.991141189894104 | 0.9885753490753 |
肽段表
| 肽段序列 | 修饰 | 后验错误率（越低越好） | q值（越低越好） | 归属的蛋白group数目 | 归属的蛋白数目 | 二级谱图数目（肽段谱图匹配数目，越多越好） | 主蛋白识别号 | 漏切位点 | 分子量（理论） | 肽段可信度等级 | sequest搜索引擎得到的p值 | sequest搜索引擎得到的后验错误率值 | 实际检测的质谱谱图与理论谱图比较计算的得分值（越高越好） |
|---|
| Annotated Sequence | Modifications | Qvality PEP | Qvality q-value | # Protein Groups | # Proteins | # PSMs | Master Protein Accessions | # Missed Cleavages | Theo. MH+ [Da] | Confidence Sequest HT | Percolator q-Value Sequest HT | Percolator PEP Sequest HT | XCorr Sequest HT |
DEqMS
https://evvail.com/2020/07/26/1001.html
