差异蛋白分析

最后发布时间 : 2023-02-21 23:48:50 浏览量 :

蛋白质一级结构是组成蛋白质多肽链的线性氨基酸序列。蛋白测序，蛋白序列分析，蛋白一级结构分析，蛋白氨基酸序列分析是确定蛋白质全部氨基酸序列的过程。通过蛋白质测序获得的信息，有非常多有价值的用途，包括：

用于蛋白质的鉴定；
设计分子克隆的探针；
合成可用作免疫原的肽段等。

蛋白测序服务常见有两种主要的方法：Edman降解（Harvey和Ferrier，2011； Bauer等，1997）和质谱分析（MS）（Sahukar等，2016）。
目前使用最广的蛋白质测序和鉴定方法是质谱法，而Edman降解则是表征蛋白质N端最重要的方法之一。

PSMs:Displays the number of identified peptide spectrum matches identified from all included searches, including those redundantly identified.

蛋白表

主蛋白的识别号	基因名称	蛋白名称的详细描述	肽段覆盖率	鉴定到不同肽段的总数目	肽段匹配到二级谱图的数目	unique肽段数目	归属的蛋白group数目	氨基酸残基数	蛋白的分子量	蛋白的等电点	定量值	Sequest程序打分
Accession	Gene Name	Description	Coverage	# Peptides	# PSMs	# Unique Peptides	# Protein Groups	# AAs	MW [kDa]	calc. pI	Area: F1: Sample	Score Sequest HT

Protein accession	Gene	Associated gene ID	Description	Coverage	# Proteins	Proteins in group	miR FASP_# Unique peptides	miR FASP_# Peptides	miR FASP_# PSMs	miR FASP_Protein error probability	miR FASP_q-value	miR FASP_PEP	miR FASP_MS1 precursor area	miR FASP_tmt10plex_126	miR FASP_tmt10plex_126 - # quanted PSMs	miR FASP_tmt10plex_127N	miR FASP_tmt10plex_127N - # quanted PSMs	miR FASP_tmt10plex_127C	miR FASP_tmt10plex_127C - # quanted PSMs	miR FASP_tmt10plex_128N	miR FASP_tmt10plex_128N - # quanted PSMs	miR FASP_tmt10plex_128C	miR FASP_tmt10plex_128C - # quanted PSMs	miR FASP_tmt10plex_129N	miR FASP_tmt10plex_129N - # quanted PSMs	miR FASP_tmt10plex_129C	miR FASP_tmt10plex_129C - # quanted PSMs	miR FASP_tmt10plex_130N	miR FASP_tmt10plex_130N - # quanted PSMs	miR FASP_tmt10plex_130C	miR FASP_tmt10plex_130C - # quanted PSMs	miR FASP_tmt10plex_131	miR FASP_tmt10plex_131 - # quanted PSMs
A2M	NA	ENSG00000175899	alpha-2-macroglobulin [Source:HGNC Symbol;Acc:HGNC:7]	NA	na	NA	8	9	12	2.874853755573355e-38	0.0	0.00162245	16352434.333333334	0.930830807074245	11	0.772021464969435	11	0.707903988854009	11	0.789387195411552	11	1.15137143542937	11	0.826996046675429	11	0.760178732847342	11	0.91701736567271	11	0.854543633421946	11	0.603476404140484	11
A2ML1	NA	ENSG00000166535	alpha-2-macroglobulin-like 1 [Source:HGNC Symbol;Acc:HGNC:23336]	NA	na	NA	1	1	1	0.0169213	0.0141899	0.7890064999999999	768887.0	0.887330152432326	1	1.09479780129344	1	1.0157434594774	1	1.29660702438037	1	0.973056657814277	1	0.997696101059528	1	1.26868409705732	1	1.13317687425116	1	1.33611556782666	1	1.12558143277205	1
AAAS	NA	ENSG00000094914	achalasia, adrenocortical insufficiency, alacrimia [Source:HGNC Symbol;Acc:HGNC:13666]	NA	na	NA	6	6	11	8.178128730099644e-39	0.0	0.00162245	69955068.0	0.915436990896682	11	0.916836199551183	11	0.941698838939896	11	0.88544134353248	11	0.965729126460487	11	0.847425426889061	11	0.904473477822955	11	1.14627079478433	11	0.92015871221395	11	1.019089204302	11

gene	miR.FASP_tmt10plex_126	miR.FASP_tmt10plex_127N	miR.FASP_tmt10plex_127C	miR.FASP_tmt10plex_128N	miR.FASP_tmt10plex_128C	miR.FASP_tmt10plex_129N	miR.FASP_tmt10plex_129C	miR.FASP_tmt10plex_130N	miR.FASP_tmt10plex_130C	miR.FASP_tmt10plex_131
A2M	0.930830807074245	0.772021464969435	0.707903988854009	0.789387195411552	1.15137143542937	0.826996046675429	0.760178732847342	0.91701736567271	0.854543633421946	0.603476404140484
AAAS	0.915436990896682	0.916836199551183	0.941698838939896	0.88544134353248	0.965729126460487	0.847425426889061	0.904473477822955	1.14627079478433	0.92015871221395	1.019089204302
AACS	0.96245526191104	0.947590854699577	1.01977053925564	0.964200014652402	0.963690875567279	0.887846978098863	1.00208476389661	1.06062507803835	0.953661694404969	0.950616066145275
AAED1	0.900508679946027	1.10751066220758	1.31041214292136	1.11527207267666	1.01450612064567	1.07518082408031	1.02966783708056	1.06466009392292	1.0366690039719	1.24507134739029
AAGAB	1.07230101858693	1.2126029407603	0.993777999029608	1.05762971893229	1.01511504613838	1.0637392532722	0.912021160518172	0.912052854266589	1.0656559070064	1.08498261123753
AAK1	1.01350831210127	0.98433193366552	1.02797359945557	1.14908644786092	0.986735632115981	1.03463177873044	1.1678986196205	1.02933866306218	0.991141189894104	0.9885753490753

肽段表

肽段序列	修饰	后验错误率（越低越好）	q值（越低越好）	归属的蛋白group数目	归属的蛋白数目	二级谱图数目（肽段谱图匹配数目，越多越好）	主蛋白识别号	漏切位点	分子量（理论）	肽段可信度等级	sequest搜索引擎得到的p值	sequest搜索引擎得到的后验错误率值	实际检测的质谱谱图与理论谱图比较计算的得分值（越高越好）
Annotated Sequence	Modifications	Qvality PEP	Qvality q-value	# Protein Groups	# Proteins	# PSMs	Master Protein Accessions	# Missed Cleavages	Theo. MH+ [Da]	Confidence Sequest HT	Percolator q-Value Sequest HT	Percolator PEP Sequest HT	XCorr Sequest HT

DEqMS
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