公共数据库测序数据获取
最后发布时间:2022-03-01 09:34:10
浏览量:
测序数据分析流程
序列的比对和组装
定量和标准化
差异分析
TCGA数据下载
网页下载
Study Abbreviation | Study Name | 翻译 |
---|---|---|
TCGA-LAML | Acute Myeloid Leukemia | 急性髓性白血病 |
TCGA-ACC | Adrenocortical carcinoma | 肾上腺皮质癌 |
TCGA-BLCA | Bladder Urothelial Carcinoma | 膀胱尿路上皮癌 |
TCGA-LGG | Brain Lower Grade Glioma | 脑低级脑胶质瘤 |
TCGA-BRCA | Breast invasive carcinoma | 乳腺浸润癌 |
TCGA-CESC | Cervical squamous cell carcinoma and endocervical adenocarcinoma | 宫颈鳞状细胞癌和宫颈内膜腺癌 |
TCGA-CHOL | Cholangiocarcinoma | 胆管癌 |
TCGA-LCML | Chronic Myelogenous Leukemia | 慢性粒细胞性白血病 |
TCGA-COAD | Colon adenocarcinoma | 结肠腺癌 |
TCGA-ESCA | Esophageal carcinoma | 食道癌 |
TCGA-GBM | Glioblastoma multiforme | 胶质母细胞瘤 |
TCGA-HNSC | Head and Neck squamous cell carcinoma | 头颈部鳞状细胞癌 |
TCGA-KICH | Kidney Chromophobe | 肾染色体 |
TCGA-KIRC | Kidney renal clear cell carcinoma | 肾脏肾透明细胞癌 |
TCGA-KIRP | Kidney renal papillary cell carcinoma | 肾肾乳头状细胞癌 |
TCGA-LIHC | Liver hepatocellular carcinoma | 肝肝细胞癌 |
TCGA-LUAD | Lung adenocarcinoma | 肺腺癌 |
TCGA-LUSC | Lung squamous cell carcinoma | 肺鳞状细胞癌 |
TCGA-DLBC | Lymphoid Neoplasm Diffuse Large B-cell Lymphoma | 淋巴样肿瘤弥漫性大B细胞淋巴瘤 |
TCGA-MESO | Mesothelioma | 间皮瘤 |
TCGA-OV | Ovarian serous cystadenocarcinoma | 卵巢浆液性囊腺癌 |
TCGA-PAAD | Pancreatic adenocarcinoma | 胰腺腺癌 |
TCGA-PCPG | Pheochromocytoma and Paraganglioma | 嗜铬细胞瘤和副神经节瘤 |
TCGA-PRAD | Prostate adenocarcinoma | 前列腺腺癌 |
TCGA-READ | Rectum adenocarcinoma | 直肠腺癌 |
TCGA-SARC | Sarcoma | 肉瘤 |
TCGA-SKCM | Skin Cutaneous Melanoma | 皮肤皮肤黑色素瘤 |
TCGA-STAD | Stomach adenocarcinoma | 胃腺癌 |
TCGA-TGCT | Testicular Germ Cell Tumors | 睾丸生殖细胞肿瘤 |
TCGA-THYM | Thymoma | 胸腺瘤 |
TCGA-THCA | Thyroid carcinoma | 甲状腺癌 |
TCGA-UCS | Uterine Carcinosarcoma | 子宫癌肉瘤 |
TCGA-UCEC | Uterine Corpus Endometrial Carcinoma | 子宫体子宫内膜癌 |
TCGA-UVM | Uveal Melanoma | 葡萄膜黑色素瘤 |
R包
TCGA一般下载那种数据比较好呢?
如果是做差异分析的话,我建议采用counts ,毕竟有不少的差异分析的软件都是基于counts数。
如果是计算样品间的相关性,聚类等,那就可以采用均一化的FPKM,和FPKM-UQ。当然下载counts,之后进行标准化,也是可以的。
下载数据后,在数据分析时,用的方法也是不同的,Counts数据一般使用edgeR包或DESeq包,对数据做分析;如果下载FPKM数据,就不能使用edgeR包,只能只用DESeq包进行处理。
TARGET数据下载
GTEx数据下载
- Genotype-Tissue Expression
- 均为捐赠者的正常样本
TCGA和GTEx
参考
RNA sequencing: the teenage years
https://blog.csdn.net/weixin_43960055/article/details/109553673